Research Associate I/II
Join the Zhang Lab: an interdisciplinary team of scientists developing and applying technologies for genome and transcription engineering. Contribute to large-scale experiments designed to identify new candidate genes relevant to cellular function and disease phenotypes.Work as part of a small team to execute high-throughput lentiviral genome-wide CRISPR/Cas9 screens, including virus production and transduction of cultured cells.
Responsibilities include: cell culture, screening protocols, next-generation sequencing prep, and data analysis.
- Execute high-throughput genome-wide lentiviral screens.
- Perform cell culture on many parallel cell lines, at high capacity.
- Lentivirus production, titration, and resistance selection for transduction.
- Assist in a variety of molecular reagent preparations including: next-generation sequencing library prep, plasmid DNA production and purification.
- Involvement in data analysis in accordance with candidate’s interest.
- Significant experience with mammalian tissue culture.
- Bachelor’s degree in a life science related field.
- Keen attention to detail; ability to precisely follow experimental protocols.
- Manage quantitative aspects of cell-based experiments. Examples: accurate cell plating, growth rate tracking, calculation of transduction efficiencies.
- Willingness and ability to work in a very clean tissue culture facility, such as a biosafety level 2+ lab.
- Start date no later than July 1. Immediate availability is a significant plus.
Useful skills, but not required:
- Prior experience with production, titration, and application of lentiviral vectors.
- Experience with next-generation sequencing.
- Experience with flow cytometry or FACS.
- Experience with DNA based techniques: PCR, plasmid purification, genomic DNA purification.
- Experience with RNA purification and qPCR.
- Experience with Excel and Matlab data analysis.Interested candidates should send a cover email and CV/resume to firstname.lastname@example.orgThe Broad will not offer visa sponsorship for this position.
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