Cleavage, deprotection and purification of synthetic oligoribonucleotides, although routine, are complex processes. No two labs seem to carry out the procedures the same way and there are many
routes to successful completion of the tasks. In the procedures here, we assume that cleavage and deprotection will occur off the synthesizer and that our TBDMS protected RNA monomers are used to manufacture the oligoribonucleotide.
Procedure for the synthesis, deprotection and isolation of RNA using TBDMS-protected monomers
Jul 25, 2019
Application Guide